One. Why study sleep <br> Sleep is an important physiological process that maintains physical, mental and emotional well-being. Therefore, sleep deprivation (SD) is associated with adverse outcomes and increases the risk of anxiety, immune and cognitive impairment. SD is characterized by increased energy consumption response and post-recovery sleep bounce, which is regulated by a steady state process, which in turn is subject to stress.
two. Strategies for assessing sleep homeostasis <br> Sleep deprivation (SD) is a common method strategy that allows for a differential assessment of sleep homeostasis, leading to “sleep debtâ€.
three. Why use deprivation-type sleep deprivation <br> There are many ways to deprive sleep, including slow-rotating wheel sleep deprivation, water platform sleep deprivation, and treadmill sleep deprivation. Most of these techniques are stress-induced, and stress levels may be alleviated even after long-term adaptation to this method. After long-term sleep deprivation, the animals showed a clear physiological sleep deprivation syndrome, with significant interference from the release. For these reasons, we have implemented a novel sleep deprivation instrument that uses a gap deprivation bar scan in a standard laboratory cage to perform tactile stimulation to interfere with free-behaving mice. This approach prevents the effects of human exposure and intervention on sleep deprivation and minimizes physical activity throughout the sleep deprivation process. In addition, the program is accurate and predictable.
In addition, restrained cables used to record electroencephalogram (EEG) and electromyography (EMG) can cause sustained stress. In fact, a recent study showed that cable weight and flexibility may affect the amount and pattern of sleep in mice. The development of telemetry systems not only provides a stress-free environment, but also provides an opportunity to study the effects of social interaction on sleep in mice.
four. Why standard-based laboratory cages <br> Laboratory animals have grown up in cages since childhood, and based on laboratory cages, animals have been able to maintain a relatively stress-free approach to induce animals in a normal social setting. The sleep deprivation model avoids the effects of the external environment and other factors on sleep deprivation.
In addition, the cage-based sleep deprivation instrument is easy to assemble and clean, and the design is more user-friendly. 
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