1. Cut (cut) tail blood collection This method is used when the required blood volume is small. Fix the animal and expose the tail. The tail hair is cut and disinfected, and then immersed in warm water of about 45 ° C for a few minutes to fill the tail blood vessels. Then dry the tail, cut off the tip of the tail with a sharp knife (knife or scissors) 0.3 ~ 0.5cm, let the blood drop freely into the container or use a hemoglobin pipette to absorb, blood collection ends, the wound disinfection and pressure to stop bleeding. A transverse incision can also be made in the tail to cut the tail artery or vein and collect blood as above. Each mouse can generally collect more than 10 times of blood. The mouse can take 0.1 ml of blood each time, and the rat has 0.3 to 0.5 ml. Â
2. Rat tail spurting method When the rats use less blood (only white blood cell count or hemoglobin test), this method can be used. First wipe the rat tail with warm water, then disinfect and wipe with alcohol to make the tail of the mouse congestion. Use the No. 7 or No. 8 injection needle to puncture the rat tail vein. When the needle is pulled out, blood will drip out, and 10 to 50 mm3 can be collected at one time. If the blood is taken repeatedly for a long time, it should be puncture near the end of the rat tail, and then gradually puncture to the proximal end. Â
3. Eyelid venous plexus blood collection Â
The blood donor's left thumb and the two fingers hold the neck of the mouse or rat tightly from the back (the rat needs to wear a pair of gauze gloves), and the animal should be prevented from suffocating. When taking blood, the left thumb and forefinger are gently Press the sides of the neck of the animal to make the posterior venous plexus congestive. The right hand is connected to the 1ml syringe of the 7th needle or the long neck (3~4cm) hard glass dropper (capillary inner diameter 0.5~1.0mm) to make the blood collection device The rat's surface is at an angle of 45 °C, which is pierced by the inner corner of the eye. The oblique surface of the needle is first directed to the eyeball. After the penetration, it is rotated 180 degrees to make it obliquely facing the posterior border of the eyelid. The concentration of the needle is about 2 to 3 mm, and the rat is about 4 to 5 mm. When there is resistance, stop pushing. At the same time, the needle is withdrawn from about 0.1 to 0.5 mm, and the side is withdrawn. If the appropriate blood is puncture, it can naturally flow into the capillary. When the required blood volume is obtained, the needle is removed. Add pressure to the neck, at the same time, pull out the blood collection device to prevent postoperative puncture hole bleeding. If skilled, it is not difficult to repeat blood collection in this short-term method. The left and right eyes are better rotated. Weight 20~ 25g of mice can collect 0.2-0.3ml of blood each time; rats weighing 200-300g can be harvested each time. Blood 0.5 ~ 1.0ml, can be applied to the inspection of certain biochemical projects. Â
4. Decapitated blood Â
The blood donor's left thumb and forefinger hold the neck of the large (small) mouse tightly with the back and make the animal's head tilt down. The right hand uses a pair of scissors to sharply cut the mouse neck, about 1/2 to 4/5 of the neck before cutting, allowing blood to drop freely into the container. The mouse can be used in an amount of about 0.8 to 1.2 ml; the rat is about 5 to 10 ml. Â
5. Heart blood sampling Â
The heart of the mouse is small, and the heart rate is faster. It is difficult to collect blood from the heart, so it is rarely used. The method of collecting blood in vivo is the same as that of guinea pigs. If you do a blood-throwing death, first deep-anesthetize the animal, open the chest, expose the heart, puncture the right ventricle with a needle, and draw blood. The mouse is about 0.5 to 0.6 ml; the rat is about 0.8 to 1.2 ml. Â
6. Carotid artery blood collection Â
The animal is fixed in the up position, the neck skin is cut, the subcutaneous connective tissue is separated, the jugular vein is fully exposed, and the blood can be aspirated by a syringe. in Â
The carotid artery was separated on both sides of the trachea, the end of the centrifuge was ligated, and the blood was cut into the test tube. Â
7. Abdominal aorta blood collection Â
It is best to anesthetize the animal first, fix it on the surgical frame on the back, and cut the abdominal cavity from the median line of the abdomen to make the abdominal aorta clearly exposed. Use a syringe to aspirate the blood to prevent hemolysis. Or use the toothless tweezers to peel off the connective tissue, clamp the proximal end of the artery, and use a pointed surgical scissors to cut the artery and spray the blood into the container. Â
8. Stock movement (static) pulse blood collection Â
The animal is first held by the assistant, and the blood donor pulls the lower limb of the animal to the left to fill the vein. Or use the pulse as an indicator, and the right hand pierces the blood vessel with a syringe. The mice weighing 15-20 g of blood collected about 0.2-0.8 ml, and the rats about 0.4-0.6 ml. Â
2. Rat tail spurting method When the rats use less blood (only white blood cell count or hemoglobin test), this method can be used. First wipe the rat tail with warm water, then disinfect and wipe with alcohol to make the tail of the mouse congestion. Use the No. 7 or No. 8 injection needle to puncture the rat tail vein. When the needle is pulled out, blood will drip out, and 10 to 50 mm3 can be collected at one time. If the blood is taken repeatedly for a long time, it should be puncture near the end of the rat tail, and then gradually puncture to the proximal end. Â
3. Eyelid venous plexus blood collection Â
The blood donor's left thumb and the two fingers hold the neck of the mouse or rat tightly from the back (the rat needs to wear a pair of gauze gloves), and the animal should be prevented from suffocating. When taking blood, the left thumb and forefinger are gently Press the sides of the neck of the animal to make the posterior venous plexus congestive. The right hand is connected to the 1ml syringe of the 7th needle or the long neck (3~4cm) hard glass dropper (capillary inner diameter 0.5~1.0mm) to make the blood collection device The rat's surface is at an angle of 45 °C, which is pierced by the inner corner of the eye. The oblique surface of the needle is first directed to the eyeball. After the penetration, it is rotated 180 degrees to make it obliquely facing the posterior border of the eyelid. The concentration of the needle is about 2 to 3 mm, and the rat is about 4 to 5 mm. When there is resistance, stop pushing. At the same time, the needle is withdrawn from about 0.1 to 0.5 mm, and the side is withdrawn. If the appropriate blood is puncture, it can naturally flow into the capillary. When the required blood volume is obtained, the needle is removed. Add pressure to the neck, at the same time, pull out the blood collection device to prevent postoperative puncture hole bleeding. If skilled, it is not difficult to repeat blood collection in this short-term method. The left and right eyes are better rotated. Weight 20~ 25g of mice can collect 0.2-0.3ml of blood each time; rats weighing 200-300g can be harvested each time. Blood 0.5 ~ 1.0ml, can be applied to the inspection of certain biochemical projects. Â
4. Decapitated blood Â
The blood donor's left thumb and forefinger hold the neck of the large (small) mouse tightly with the back and make the animal's head tilt down. The right hand uses a pair of scissors to sharply cut the mouse neck, about 1/2 to 4/5 of the neck before cutting, allowing blood to drop freely into the container. The mouse can be used in an amount of about 0.8 to 1.2 ml; the rat is about 5 to 10 ml. Â
5. Heart blood sampling Â
The heart of the mouse is small, and the heart rate is faster. It is difficult to collect blood from the heart, so it is rarely used. The method of collecting blood in vivo is the same as that of guinea pigs. If you do a blood-throwing death, first deep-anesthetize the animal, open the chest, expose the heart, puncture the right ventricle with a needle, and draw blood. The mouse is about 0.5 to 0.6 ml; the rat is about 0.8 to 1.2 ml. Â
6. Carotid artery blood collection Â
The animal is fixed in the up position, the neck skin is cut, the subcutaneous connective tissue is separated, the jugular vein is fully exposed, and the blood can be aspirated by a syringe. in Â
The carotid artery was separated on both sides of the trachea, the end of the centrifuge was ligated, and the blood was cut into the test tube. Â
7. Abdominal aorta blood collection Â
It is best to anesthetize the animal first, fix it on the surgical frame on the back, and cut the abdominal cavity from the median line of the abdomen to make the abdominal aorta clearly exposed. Use a syringe to aspirate the blood to prevent hemolysis. Or use the toothless tweezers to peel off the connective tissue, clamp the proximal end of the artery, and use a pointed surgical scissors to cut the artery and spray the blood into the container. Â
8. Stock movement (static) pulse blood collection Â
The animal is first held by the assistant, and the blood donor pulls the lower limb of the animal to the left to fill the vein. Or use the pulse as an indicator, and the right hand pierces the blood vessel with a syringe. The mice weighing 15-20 g of blood collected about 0.2-0.8 ml, and the rats about 0.4-0.6 ml. Â
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