The strain production process is usually divided into three levels: parent species, primary species, and cultivated species, which are commonly referred to as primary, secondary, and tertiary bacteria. Among them, the separation and breeding of parent species is highly technical and requires refinement; while the production of original species and cultivars is relatively simple. The following describes the production techniques of various strains.
The method of separation and breeding of female parent is: the female parent is mainly obtained by artificial selection, mutation breeding, cross breeding and protoplasm fusion. As a general seed production professional household, the mother seed can be isolated and cultivated manually. The specific steps and operation methods are as follows.
1. Collect seed sources. From the wild or artificially cultivated population, select representative elite mushrooms are used as provenances. 1 kind of mushroom selection time. Usually in the middle of November, the first and second batch of mushrooms are selected. Because the mushroom growth during this time is very strong, the mycelium has a strong life force, robust fruit bodies, and good quality. In addition, the temperature is appropriate and the mushrooms can mature normally. The standard of the two kinds of mushrooms is: Eight mature, the flower shape is round, the flesh is hypertrophic, the color is white, no pests and diseases. One or two of the mushrooms that meet the above criteria were numbered and used as the material for the separation of the mother plants. Collected from the cultivation room should be marked with the original strain code.
2. Medium preparation. The agar medium is also called PDA medium. The commonly used formula is: 200 g of potato, 16-18 g of agar, 20 g of glucose, and 1000 ml of water. The potatoes are washed and peeled, cut into thin slices, placed in an aluminum pan and boiled for 15 to 30 minutes until crispy potatoes are not rotten. After boiling, use 6 to 8 layers of gauze soaked in advance to wrap the mixture. Add 16-18 g of agar after filtration (add 16 g at low temperatures and add 18 g at high temperatures) and cook. Stir constantly and stir the solid agar completely. Dissolve and filter with 4-6 layers of gauze. The filtrate was added with water to 1000 ml, 20 g of glucose was added, and the mixture was dissolved in a hot melt and aliquoted into a test tube. The loading amount is 1/4 to 1/5 of the height of the test tube. Care should be taken not to stick the test tube to the culture medium. The tube should be plugged with a tampon or the juice should be placed in a glass flask with a volume of 20 ml. Then placed in a pressure cooker, the pressure of 108 kPa, the pot temperature of 121 ° C sterilization for about 30 minutes. After being sterilized, they are immediately removed and the tube is slanted on the table while it is hot. After cooling, it becomes a solid slant medium.
As people pay more attention to the concept of health, nature, and non-hazard, the more people demand pure natural plants in their daily lives.
Pure plant powder is a natural plant raw meal or slice made of natural plants through physical processing (crushing, shearing, grinding). No additives are added during the production process, and the original effective ingredients of the plant are retained.
According to different market needs, it can be made into tea cuts,Slice,TBC, plant powders, and applied to tea bags, health products, etc. Common plant powders include Astragalus powder, Reishi Mushroom powder, Pueraria lobata root powder, Moringa leaf powder and other plant powders.
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