1. When cultivating bacterial material, it's essential to carefully examine the bacteria and bacterial rods. If mixed fungus and rot are present, students should use non-corrosive, mold-free fresh wood for cultivation and minimize the culture time as much as possible. If bacteria are found on the rods, lightly infested ones should be scraped off and dried for 1–2 days. Severely infected rods should be discarded to prevent contamination.
2. It's important to check the type of hemp being used. If mildew is detected, the hemp should be lightly shaved, exposed to sunlight, or partially disinfected using 75% alcohol or carbendazim. Any damaged or contaminated hemp must be discarded immediately to avoid spreading infection.
3. Ensure that the filling is compact and free of gaps. Proper management of temperature, humidity, and air circulation is crucial. Controlling the moisture levels inside the growing medium helps maintain a dry environment, which significantly reduces the risk of mold development.
4. The Tianma points should not be too large or too deep. Using a small number of bacterial sticks in bacillus cultivation is the most effective method to prevent bacterial infections. This approach allows better control over the growth conditions and minimizes the chances of unwanted microbial contamination.
5. Increasing the amount of honey fungus can help promote faster bacterial growth. By introducing more bacteria or strains, you can create a competitive environment that suppresses the growth of harmful microorganisms. This technique not only speeds up the process but also enhances the overall quality of the culture.
By following these detailed steps, growers can significantly improve the success rate of their bacterial cultures while maintaining a clean and controlled environment. Attention to detail at each stage is key to achieving optimal results and avoiding common issues like mold and contamination.
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