1. When cultivating bacterial material, it's essential to carefully inspect the bacteria and bacterial rods. If mixed fungi and rot are present, students should use non-corrosive, mold-free fresh wood for cultivation and keep the culture period as short as possible. If bacteria are found on the rods, gently scrape off the lighter ones and dry them for 1–2 days. Severely contaminated rods should be discarded immediately to prevent further spread.
2. It's important to check the type of hemp being used. If mildew is detected, lightly shave the affected areas, expose them to sunlight, or apply 75% alcohol or carbendazim for partial disinfection. Any damaged or compromised hemp must be completely discarded to avoid contamination in the cultivation process.
3. Ensure that the filling is compact and free of gaps. Proper management of temperature, humidity, and air circulation is crucial. Controlling the moisture inside the container and maintaining a dry environment can significantly reduce the risk of mold development.
4. The size and depth of the Tianma points should be moderate. Using a small number of bacterial sticks with bacillus cultivation is considered the most effective method to prevent bacterial infections and maintain a healthy culture environment.
5. Increasing the amount of honey fungus can help promote faster bacterial growth. By introducing more bacteria or strains, the beneficial microorganisms can outcompete harmful ones, creating a more stable and controlled cultivation system.
Overall, attention to detail in each step of the process—from selecting materials to managing environmental conditions—is key to successful bacterial cultivation and minimizing contamination risks.
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