1 Choosing sheep for donors and acceptors 1.1 Donor goats adopting embryo transfer should be excellent sheep with good performance, high production level, and stable genetics. Age 2 to 5 years old, normal reproductive history, normal estrus cycle, healthy and disease-free, especially in the absence of reproductive tract disease. Non-transgenic lambs are usually not suitable due to poor superovulation, but well-developed individuals over the age of one can also choose to use it. In ewes older than 6 years old, their ovarian function deteriorated due to reduced feeding capacity and physical fitness, and the number and quality of their embryos were lower than those of young adults. Therefore, it is not used under normal circumstances, except for individuals with good physical and reproductive performance. The donor sheep can be reused 2-3 times, depending on the success of the egg-sweeping operation. If the operation is skillful and the genital tract of the sheep is not severely affected by infection, it can be used for the second and third time. Super-disposal treatment, after the super-disposal treatment, has produced one sheep normally and is safer. 1.2 Recipient sheep Regardless of the effect of estrus treatment in the same period, or from the lamb's birth weight and growth and development, the body size, high milk production, health and disease-free, 2-4 births lamb history 2-4 Aged young and young dairy goats are ideal Boer goat embryonic transplant recipients, while aging sheep and single-tyred goats are less effective. The plan for supplying and accepting sheep is suitable for 1:12 to 1:13. 2 Strengthen the feeding and management of the supply and acceptor sheep for the feeding and management of the recipient sheep and the requirements for the normal reproduction of the production of ewes are basically the same. The sheep were required to be in good health, with medium and high sensation. Being too fat or too thin can lead to irritability or ovulation. Suddenly changing the feeding and management environment, changing feeding methods, feed, and long-distance transportation. Accidental shocks and the occurrence of illnesses all present stress reactions. The stress response directly affects the donor's superovulation effect, manifested as: no ovulation, low ovulation or embryo degeneration, death. Recipients with stress response showed: Irregular estrus, degeneration of the corpus luteum, and low rate of conception. Therefore, the sooner and better the purchase of the donor and acceptor sheep for embryo transfer, gradually adapts to the environment in which it is raised, and pays attention to the coordination and modulation of various nutrient components in the feed. Selenium-deficient sheep should also be injected with sodium selenite vitamin E injection every six months. All vaccination and deworming work is completed before the concurrent clearing process. Avoid all stress reactions as much as possible. 3 Selecting the appropriate seasonal Boer goats and Guanzhong dairy goats have good reproductive performance. They produce twin lambs or multiple lambs. They can be estrus and breed all year round, but the breeding time is mainly concentrated in the autumn, followed by the spring. In order to make the best use of the potential natural reproductive performance of sheep and achieve the high benefits of embryo transfer, Boer goat embryo transplantation in the northern region is best performed in autumn, and spring can also be selected. In addition to the hot summer in the south, other seasons can be carried out. 4 Selecting Reliable Hormone Drugs and Appropriate Treatment Methods At present, domestic hormonal drugs used for estrus and superovulation in animals at the same time are domestically produced and imported. From the use of results, domestic drugs, although the price is lower, but the quality of the batch is quite different, each batch of drugs need to be treated before use. Imported drug performance is relatively stable, but the price is higher. Everyone should choose according to the specific circumstances. Supersaturation of the donor sheep is ideal for the administration of follicle stimulating hormone (FSH). In the treatment, decremental hip injection is used. This method is more troublesome to perform. For this reason, it has been reported that a full amount of intramuscular injection of FSH diluted with 15% to 30% polyvinylpyrrolidone (PVP) solution has the same effect of superovulation as multiple injections, which greatly simplifies the procedure of superovulation. Reciprocal estrus treatment of the recipient sheep was better with the combination of vaginal suppository (CID-G) and pregnant horse serum (PMSG). 5 Pay attention to the mating of donor sheep and the record of the recipient sheep. This work relates to the quality of embryos and the effect of transplantation. The late and premature mating of the donor sheep results in the aging of the egg and sperm. The aging eggs and sperm are not conducive to fertilization. Even if fertilized, the embryo can not develop normally, resulting in early degeneration and death, resulting in low conception rate. The rams will conduct training and check the quality of semen 1-2 months before use. Rams used for mating with donor sheep should select individuals with superior semen quality, energy, and disease-free health. They should not use rams and rams that are untrained, have poor semen quality, are sluggish or overused. The donor sheep started from the crawler and was assigned every 8h until the end of the estrus. In general, a donor sheep needs 4-6 times. If the ram is insufficient, the donor sheep can be artificially inseminated, and artificial insemination should use the same quality and fresh essence collected on the day. The input semen dose should be higher than usual. Frozen semen has a low conception rate and should not be used for mating of donor sheep. Recipient sheep should be tested on the ram that was ligated with the vas deferens, and also tested every 8 hours and recorded in detail. 6 Do a good job of pre-embryonic preparation and post-operative anti-infective work Strictly clean and disinfect the surgical instruments before surgery. The operating room, especially the embryology room, should be thoroughly cleaned and sterilized with ultraviolet light. The donor and recipient sheep were fasted 24 hours before surgery and matched according to their estrus time. Surgical staff disinfected the arms before surgery and wore aseptic workers, caps, masks, and gloves. Careful management of postoperative sheep is required. In addition to the injection of necessary anti-infective drugs, sheep should be observed at any time for feeding. Excretion, if necessary, also measures body temperature and inspects wounds. Find the infection and take immediate action. Good husbandry and management are the basic conditions for the recovery of donor reproductive function and the normal development of embryos in recipient sheep. Recipient sheep With the development of the fetus, the demand for nutrients increases, especially in the late pregnancy, not only to provide adequate nutrient-rich fine, roughage, but also pay attention to the supply of mineral elements and vitamins A, D, E, especially Carrot supplements. On the other hand, the pens should be kept clean and dry, and the rearing density should not be too large. The group structure should remain relatively stable to prevent the sheep from fighting each other and cause miscarriage.
Dermabrasion HydraFacial System
Derma Pen,Dr Pen,Skin Nursing Care
Haosheng Pneumatic Machinery Co., Ltd. , http://www.nb-tattoo.com