Hitherto, a method for producing high-concentration fructose has been carried out by using glucose glucose as a raw material and converting it into a mixture of glucose and glucose using a dedicated glucose isomerase, followed by separation to yield glucose. This isomerase method has the following two major problems that cannot be solved: 1. Due to the reaction equilibrium point of glucose isomerase, the yield of fructose is only 42%, and the reaction is stopped when the highest reaches 55% as the equilibrium point. 2. For this purpose, it is necessary to use a variety of methods such as an ion exchange column method to separate and remove glucose to increase the concentration of fructose, and it is costly to produce a high concentration of fructose. On the other hand, a new method of producing fructose by the oxidative fermentation of acetic acid bacteria is based on the process of starting the reduction of glucose to produce sorbitol and then converting the fructose. In the study, the raw material for the conversion of sorbitol to fructose was discovered. Now that sorbitol is manufactured, the problem of raw materials can be solved. Taking into account the sorbitol fermentation of acetic acid bacteria, sorbitol was oxidized to produce sorbose according to the principle of the Tora-la-Hamison. In this oxidative fermentation, unlike the cytoplasmic membrane-bound sorbitol dehydrogenase (S-SLDH), which is a biocatalyst PQQ, which is a secondary fermentation, the biocatalyst that converts from sorbitol to fructose is sorbitol dehydrogenase (F -SLDH). In the oxidative fermentation method using acetic acid bacteria, the most common ones are acetic acid fermentation (acetic acid production) and glucose acid fermentation (gluconic acid production). In this classical and typical microbial substance conversion process, the characteristics can be collected. In a short time when the yield is close to 100%, an oxidative fermentation product is produced. During the oxidation and fermentation of F-SLDH, the sorbitol 2-position carbon is oxidized, and the theoretical yield of fructose is 100%, and the biocatalyst during the fermentation of F-SLDH and sorbose (S-SLDH) ) There are fundamental differences. Now, it has been confirmed through research that the genetic structure, reaction products, enzyme structure, and related coenzymes and reaction mechanisms of two enzymes as symbolic markers are fundamentally different, considering that the specific activity of F-SLDH is extremely high and the stability of the enzyme is very good. The new manufacturing method of fructose is much superior to the traditional isomerase method. First of all, the oxidative fermentation method is a new fructose production method completely different from the traditionally used old method. It utilizes the characteristics of the sorbitol dehydrogenase (S LDH) of test bacteria to be classified into sorbose synthase (S-SLDH) and fructose. Generate enzymes. According to the principle of Tolto La Hadson, sugar alcohol oxidases use PQQ as a coenzyme without exception. According to the same principle of sorbitol fermentation, the biocatalyst S-SLDH is also a coenzyme with PQQ as a result of oxidative oxidation of sorbitol. The use of the well-known G. Oxydans ATCC 621 strain in the sorbitan fermentation is not possible due to the complete S-SLDH, and therefore cannot be used for fructose production. On the other hand, F-SLDH contains FAD, and a yield of 70% to 80% of fructose accumulation can be obtained in the medium. The old method using the glucose isomerase method is difficult to obtain high-concentration fructose due to the problem of the equilibrium point of the reaction. Therefore, the oxidase method is superior to the old method in the production of high-concentration fructose. Once the practical application of the new fructose production method is successful, the technical and economic bottleneck of low yield of fructose, low production efficiency and high production cost of the glucose isomerase method has been completely solved, and it has become a new method for producing high concentration fructose. This is an epoch-making innovative technology for both the intake of excess calories and the prevention of obesity and the promotion of self-sufficiency in the production of low-priced natural sweeteners in developing countries. Its worldwide significance is enormous. By comparison, it can be seen that the yield of fructose by glucose isomerase method is about 50%. With the new method of acetic acid bacteria oxidative fermentation, the yield of fructose can reach nearly 100%.
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