First of all, on the construction and use of the tissue culture room. Today, there are quite a lot of tissue culture rooms built around the mainland of the motherland. If they are engaged in the production of butterfly orchid seedlings, they can use commissioned or self-built methods. According to my experience, if it is commissioning, the two parties must sign the contract and expressly request it. For example, it is required that the foundry provide a multiplication report every month. On the one hand, it is necessary to understand the situation in a timely manner to control the output. On the other hand, it is also necessary to know whether the foundry worker is producing as scheduled and supervise the progress. When selecting a foundry unit, it is also necessary to examine whether the foundry's technology has passed the technology and how good the credibility is.
If it is to prepare the self-built enterprise culture room, it is necessary to first consider whether the seedlings have a stable market before construction. The second is that if the seed production can reach 1 million a year, it is worthwhile to build a tissue culture room. How many operating stations are needed in particular can be calculated by the following method: One station can calculate 60,000 seedlings a year in one shift; if it is two shifts, it can be calculated by 100,000 seedlings.
One of the key procedures for tissue culture is to obtain reproductive material from healthy, virus-free, trait-stabilized plants. Here I want to emphasize that virus detection must be performed when acquiring reproductive materials. If the virus concentration of the plant is high, even if the variety is better, it must be abandoned, otherwise it will bring serious harm. As for virus detection, companies can purchase virus detectors themselves, such as CPR virus detectors or ELISAs, which are relatively costly; they can also be tested in units that have virus detection equipment. At the same time, it is best to detect 1 to 2 more times in the proliferation process.
Companies engaged in breeding work and seedling production are better able to build their own virus-free parental rooms. This is very important. Check from the source to minimize the chance of plant infection. According to my experience, install 32-mesh insect screens, exhaust fans, and other pages in the air intake and air outlet areas of the parent's room, and apply butter to insects where they may be able to drill holes to prevent insects from entering. When personnel enter the parent room, they must be rigorously managed. Air showers should be used and disinfectant should be used to disinfect; wear disinfected shoes, clothing and gloves; disposable materials should be used for cutting materials.
With regard to the use of reproductive materials, I have two suggestions: First, it is best not to use a plant material for more than 2 years, or up to 12 generations. Second, the proliferation of a bud is kept within 2,000 buds. The seedlings produced by tissue culture have low mutation rate, strong disease resistance, and stable genetic genes. In the past, there have been cases in the market where some manufacturers rushed to launch new varieties or were tempted by market demand. With a small amount of propagation material, they overproliferated in a short period of time, resulting in poor performance of the products produced, causing irreparable as a result of. These are all lessons and I hope that people in the industry can alert them.
As a producer, it is necessary to understand some of the symptoms of Phalaenopsis variability in order to control the production of bad products as early as possible. Variations of Phalaenopsis have the following symptoms: variation in flower shape, variation in flower color, uneven distribution of flower color, short flowering period or flowering time, poor resistance to disease, distorted pedicel 7-shape, and yellow flower bud. Understand these circumstances, to facilitate companies to choose honest manufacturers, choose healthy seedlings.
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